The secret for best clinical practice
The knee research group of...
A column was written in the peer reviewed journal Nederlands Tijdschrift voor Geneeskunde on a...
Prof RPA Janssen MD PhD is a faculty member of the Post-Master B&SIS...
Remain up to date with all the latest knee facts via Facebook, Twitter and other social networks
Vijven van M, Groningen van B, Kimenai J, Steen van der MC, Doeselaar van M, Janssen RPA, Ito K, Foolen J
J Exp Orthop. 2020;7(1):48-2
Purpose: Upon anterior cruciate ligament (ACL) rupture, reconstruction is often required, with the hamstring tendon autograft as the most widely used treatment. Post-operative autograft remodeling enhances the risk of graft rupture, which occurs in up to 10% of the patient population, increasing up to 30% of patients aged under 20 years. Therefore, the aim of this research was to identify potential biological predictors for graft rupture, derived from patient-specific tissue remodeling-related cell properties.
Methods: Hamstring tendon-derived cells were obtained from remnant autograft tissue after ACL reconstructions, and seeded in collagen I gels on a micro-tissue platform. Micro-tissue compaction over time – induced by altering the boundary constraints – was monitored. Pro-collagen I expression was assessed using ELISA, and protein expression of tenomodulin and α-smooth muscle actin were measured using Western blot. Expression and activity of matrix metalloproteinase 2 were determined using gelatin zymography.
Results: Cells were obtained from 36 patients (aged 12-55 years). Only micro-tissues corresponding to younger patients occasionally released themselves from the constraining posts. Pro-collagen I expression was significantly higher in younger patients. Differences in α-smooth muscle actin and tenomodulin expression between patients were found, but these were age-independent. Active matrix metalloproteinase 2 expression was slightly more abundant in younger patients.
Conclusions: The presented micro-tissue platform exposed patient-specific remodeling-related differences between tendon-derived cells, with the micro-tissues that released from constraining posts and pro-collagen I expression best reflecting the clinical age-dependency of graft rupture. This platform could become a predictive tool to identify individual patients at risk for graft rupture.