Knie-artrose

Fietsen verstandig?

Feit

Bij hardlopen wordt de knie belast met 6x uw lichaamsgewicht bij elke stap

Máxima Medisch Centrum

Value-Based Health Care

ESSKA Accredited Teacher

Orthopedie Groot Eindhoven

Goede uitleg is essentieel voor juiste keuze behandeling

Meniscus-scheur

Voorste kruisbandreconstructie

Achterste kruisbandletsel

Zorg op maat

YouTube kanaal dr RPA Janssen

Internationaal wetenschappelijk onderzoek

Kruisbandoperaties knie

RPA Janssen MD PhD

Kruisbandoperaties

Waardegedreven zorg

Last van knie-artrose?

Spreekuur / Policlinique / Outpatient clinic

Nederlandse Vereniging voor Arthroscopie

Máxima Medisch Centrum

Kruisbandoperaties bij kinderen

Veel pijn bij ernstige knie-artrose?

Samen met uw arts gekozen voor een knie-operatie?

Eenzijdige knie-artrose knie en actief leven?

Máxima Medisch Centrum Eindhoven-Veldhoven

Kwaliteit van leven

Samen kiezen voor de beste behandeling

Sporten met plezier

Oplossingen voor de lange termijn

Associate Professor Knee Reconstruction

Fact

Kneecap pain is a regular occurrence in fitness knee rehabilitation

Knee osteoarthritis

Cycling wise?

Fact

When running, the knee is loaded with 6x your body weight with each step

In Gesprek Met de Opleider

Dr. RPA Janssen is uitgenodigd voor een...

RPA Janssen
9e Nationaal Coassistenten Congre, March 8-10, 2024. Egmond aan Zee, The Netherlands
Lees meer

Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome: a diagnostic in vitro tissue remodeling platform

Vijven van M, Groningen van B, Kimenai J, Steen van der MC, Doeselaar van M, Janssen RPA, Ito K, Foolen J
J Exp Orthop. 2020;7(1):48-2

Abstract

Purpose: Upon anterior cruciate ligament (ACL) rupture, reconstruction is often required, with the hamstring tendon autograft as the most widely used treatment. Post-operative autograft remodeling enhances the risk of graft rupture, which occurs in up to 10% of the patient population, increasing up to 30% of patients aged under 20 years. Therefore, the aim of this research was to identify potential biological predictors for graft rupture, derived from patient-specific tissue remodeling-related cell properties.

Methods: Hamstring tendon-derived cells were obtained from remnant autograft tissue after ACL reconstructions, and seeded in collagen I gels on a micro-tissue platform. Micro-tissue compaction over time – induced by altering the boundary constraints – was monitored. Pro-collagen I expression was assessed using ELISA, and protein expression of tenomodulin and α-smooth muscle actin were measured using Western blot. Expression and activity of matrix metalloproteinase 2 were determined using gelatin zymography. 

Results: Cells were obtained from 36 patients (aged 12-55 years). Only micro-tissues corresponding to younger patients occasionally released themselves from the constraining posts. Pro-collagen I expression was significantly higher in younger patients. Differences in α-smooth muscle actin and tenomodulin expression between patients were found, but these were age-independent. Active matrix metalloproteinase 2 expression was slightly more abundant in younger patients. 

Conclusions: The presented micro-tissue platform exposed patient-specific remodeling-related differences between tendon-derived cells, with the micro-tissues that released from constraining posts and pro-collagen I expression best reflecting the clinical age-dependency of graft rupture. This platform could become a predictive tool to identify individual patients at risk for graft rupture.

Full open access article